n.免疫印迹法

We performed an immunoblot analysis to detect the protein expression in the cell lysate.
我们进行了免疫印迹分析，以检测细胞裂解液中的蛋白质表达。
The results showed a specific band at 55 kDa on the immunoblots, indicating the presence of the target protein.
结果显示免疫印迹上有一个在55千道尔顿的特异性强的条带，这表明目标蛋白质的存在。
The antigen was coated onto the nitrocellulose membrane for immunoblotting.
抗原被涂覆在硝酸纤维素膜上，用于免疫印迹实验。
The immune reaction was visualized with an antibody labeled with radioactive isotopes.
通过标记的抗体与放射性同位素结合，免疫反应在免疫印迹中得以可视化。
The relative abundance of the protein was quantified by comparing the intensities of the bands in immunoblots.
通过比较免疫印迹中各条带的强度来量化该蛋白质的相对丰度。
We used western blotting to confirm the functionality of the newly cloned gene.
我们通过 Western Blot 验证了新克隆基因的功能。
The patient's serum was tested for HIV antibodies through immunoblotting.
我们通过免疫印迹检测了患者的血清中是否含有HIV抗体。
The quality control step involved running a positive control on every immunoblot run.
质量控制步骤包括在每次免疫印迹运行时都要加入一个阳性对照。
The technique allowed us to identify and quantify specific immune responses in the patient's samples.
这项技术使我们能够识别并量化患者样本中的特定免疫应答。
Immunoblotting has become a gold standard for protein analysis in molecular biology research.
免疫印迹已经成为分子生物学研究中蛋白质分析的金标准。