连接酶

The ligase enzyme is responsible for joining DNA fragments during DNA replication.
连接酶在DNA复制过程中负责连接DNA片段。
The bacteriophage T4 ligase is commonly used in molecular biology labs for cloning purposes.
细菌噬菌体T4连接酶在分子生物学实验室中常用于克隆操作。
It catalyzes a phosphodiester bond formation between adjacent nucleotides, creating a continuous strand.
它催化相邻核苷酸间的磷酸二酯键形成，从而生成连续的链。
The ligation process often requires a specific buffer and primers to ensure accurate results.
连接过程通常需要特定的缓冲液和引物以保证结果的准确性。
The enzyme has high specificity, only joining complementary DNA sequences.
该酶具有高度特异性，只连接互补的DNA序列。
The T4 ligase is known for its ability to work with blunt-ended or cohesive ends of DNA fragments.
T4连接酶以其能处理平头或黏性末端的DNA片段而闻名。
In PCR, Taq ligase is not used as it can introduce unwanted mutations; instead, T4 DNA polymerase is employed.
在PCR中，不使用Taq连接酶，因为它可能导致不需要的突变，而是使用T4 DNA聚合酶。
Researchers use the enzyme to create recombinant DNA molecules by joining different genetic fragments.
研究人员利用这种酶将不同的遗传片段连接起来，创造出重组DNA分子。
The ligase reaction is usually monitored through changes in absorbance at a specific wavelength.
连接酶反应通常通过监测特定波长下的吸光度变化来监控。
A successful ligation will yield a DNA construct that can be further analyzed or expressed in a host organism.
成功的连接将产生可以进一步分析或在宿主生物中表达的DNA构建。